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Use of hexon as an antigen for the production of monoclonal antibodies capable of detecting multiple adenovirus types

Author:
Timoshicheva, Tatyana A., Zabrodskaya, Yana A., Ramsay, Edward, Amosova, Irina V.
Source:
Biologicals 2019 v.58 pp. 44-49
ISSN:
1045-1056
Subject:
Adenoviridae, Western blotting, antigens, enzyme-linked immunosorbent assay, fluorescent antibody technique, monoclonal antibodies
Abstract:
In this work, monoclonal antibodies to the adenovirus protein hexon were produced, and their biological and diagnostic properties were characterized. The specific activities of the new monoclonal antibodies, with respect to various adenovirus types, were studied by enzyme-linked immunosorbent assay, indirect immunofluorescence, and western blot analysis. The data demonstrate the potential of the monoclonal antibodies developed, namely 4B7 and 6B12, for use in the development of modern diagnostic assays.
Agid:
6293281