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Multiplexed detection and identification of respiratory pathogens using the NxTAG® respiratory pathogen panel
- Gonsalves, Sarah, Mahony, James, Rao, Arundhati, Dunbar, Sherry, Juretschko, Stefan
- Methods 2019 v.158 pp. 61-68
- data collection, freeze drying, microparticles, nucleic acid hybridization, nucleic acids, pathogens, reverse transcriptase polymerase chain reaction, viruses, United States
- The Luminex® NxTAG® Respiratory Pathogen Panel (NxTAG RPP) is an IVD-cleared assay for the simultaneous detection and identification of nucleic acids from 18 respiratory viruses and 2 (or 3 outside of the U.S.) atypical bacterial pathogens in nasopharyngeal swabs. Its scalability allows concurrent testing of up to 96 samples in a single batch. Nucleic acid extracted from 200 µL of raw specimen using the easyMAG® extractor is added directly to pre-plated, lyophilized bead reagents (LBRs), where multiplexed RT-PCR and hybridization to MagPlex-TAG™ microspheres occurs within a sealed reaction well using a single cycling program. Data acquisition is done on the MAGPIX® instrument which reads and sorts the reaction products directly from the sealed well following transfer of the assay plate from the thermal cycler. NxTAG is the newest innovation in bead-based nucleic acid chemistry developed by Luminex. Here we provide the detailed assay protocol and present data which describe the clinical and analytical performance characteristics of NxTAG RPP.