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Genetic analysis of the RNA polymerase II CTD in Drosophila
- Lu, Feiyue, Gilmour, David S.
- Methods 2019
- DNA-directed RNA polymerase, Drosophila, eukaryotic cells, gene expression, genetic analysis, models, mutation
- The Carboxy-terminal Domain (CTD) of RNA polymerase II (Pol II) plays essential roles in regulating gene expression in eukaryotes. Here, we describe multiple genetic approaches for studying the CTD in Drosophila that complement pre-existing molecular analyses of the Pol II CTD in other experimental models. These approaches will allow one to assess the effects of any CTD mutations in a developmentally complex organism. The approaches discussed in this work can in principle, be applied to analyze other transcription components in eukaryotes.