PubAg

Main content area

A novel approach for improving the productivity of ubiquinone-10 producing strain by low-energy ion beam irradiation

Author:
Gu, Shao-Bin, Yao, Jian-Ming, Yuan, Qi-Peng, Xue, Pei-Jian, Zheng, Zhi-Ming, Wang, Li, Yu, Zeng-Liang
Source:
Applied microbiology and biotechnology 2006 v.72 no.3 pp. 456-461
ISSN:
0175-7598
Subject:
Agrobacterium radiobacter, bacteria, cell nucleolus, cytoplasm, energy, irradiation, mutagenicity, mutants, mutation, nitrogen
Abstract:
Agrobacterium tumefaciens ATCC4452 cells were irradiated by nitrogen ion beam, a new mutagen, with energy of 10 keV and fluence ranging from 2.6x10¹⁴ ions/cm² to 6.5x10¹⁵ ions/cm². A similar “saddle shape” survival curve due to ion beam irradiation appeared again in this study. Some mutants with high yield of ubiquinone-10 were induced by ion implantation. High mutation rate and wide mutation spectrum were also observed in the experiment. These results suggested that the mutagenic effect of such low-energy ion influx into bacterium cells could result from multiple processes involving direct collision of particles with cytoplasm, nucleolus, and cascade atomic and molecular reactions due to plentiful primary and secondary particles.
Agid:
629338