PubAg

Main content area

BUD13 Promotes a Type I Interferon Response by Countering Intron Retention in Irf7

Author:
Frankiw, Luke, Majumdar, Devdoot, Burns, Christian, Vlach, Logan, Moradian, Annie, Sweredoski, Michael J., Baltimore, David
Source:
Molecular cell 2019 v.73 no.4 pp. 803-814.e6
ISSN:
1097-2765
Subject:
RNA-binding proteins, Vesiculovirus, antagonists, crosslinking, gene expression, interferons, introns, macrophages, messenger RNA, protein content
Abstract:
Intron retention (IR) has emerged as an important mechanism of gene expression control, but the factors controlling IR events remain poorly understood. We observed consistent IR in one intron of the Irf7 gene and identified BUD13 as an RNA-binding protein that acts at this intron to increase the amount of successful splicing. Deficiency in BUD13 was associated with increased IR, decreased mature Irf7 transcript and protein levels, and consequently a dampened type I interferon response, which compromised the ability of BUD13-deficient macrophages to withstand vesicular stomatitis virus (VSV) infection. Global analysis of BUD13 knockdown and BUD13 cross-linking to RNA revealed a subset of introns that share many characteristics with the one found in Irf7 and are spliced in a BUD13-dependent manner. Deficiency of BUD13 led to decreased mature transcript from genes containing such introns. Thus, by acting as an antagonist to IR, BUD13 facilitates the expression of genes at which IR occurs.
Agid:
6293562