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Potassium Uptake and Transport in Apple Roots Under Drought Stress

Author:
Qi, Jianguo, Sun, Simin, Yang, Lin, Li, Mingjun, Ma, Fengwang, Zou, Yangjun
Source:
Horticultural plant journal 2019 v.5 no.1 pp. 10-16
ISSN:
2468-0141
Subject:
H-transporting ATP synthase, Malus hupehensis, apples, drought, gene expression regulation, genes, horticultural crops, hydroponics, potassium, potassium channels, protons, root systems, roots, stress tolerance, transporters, trees, water stress
Abstract:
As one of the most important mineral nutrient elements, potassium (K+) plays an important role in many plant physiological processes and determines both the yield and quality of crops. There are two typical gene families that regulate K+ transport in higher plants, including K+ channels and K+ transporters. However, little is known about how these channels and transporters divide their work in response to drought stress. In this study, the hydroponic experiment was conducted on Malus hupehensis. The K+ content was found to decrease in response to drought stress in M. hupehensis, the aboveground decreased by 34.15% and the underground decreased by 3.97%. Meanwhile, the root morphology change was detected by scanning the root system. Under conditions of drought, the genes encoding K+ transporters were upregulated including MdCHX1.3, MdCHX4.11, MdCHX4.8, MdCHX4.9, MdHKT1, and MdHAK3.2. The net influx of K+ was inhibited by 19.47% with the action of K+ channel inhibitors (CsCl), however a significant decrease (80.99%; P < 0.05) was found in roots exposed to a PM H+-ATPase (orthovanadate) inhibitor by utilizing a non-invasive micro-test technique. The trend of H+ efflux was similar to that of K+. The data suggested that the positive influx of K+ through the transporter accounted for the main K+ uptake under drought stress. These results suggest that we can improve the uptake of K+ by purposely up-regulating specific K+ transporters under drought stress. This process may improve growth, yield, quality, and stress tolerance in apple trees.
Agid:
6294286