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High-level heterologous gene expression in Ochrobactrum anthropi using an A-rich UP element
- Seleem, Mohamed, Ali, Mohammed, Abd Al-Azeem, M. W., Boyle, Stephen M., Sriranganathan, Nammalwar
- Applied microbiology and biotechnology 2007 v.73 no.5 pp. 1123-1127
- DNA, DNA-directed RNA polymerase, Ochrobactrum anthropi, gene expression, genes, proteins
- DNA regions that flank a gene's promoter play an important role in determining transcription efficiency by interacting with the carboxy-terminal domain of RNA polymerase α-subunit. We placed an adenine-rich upstream element (UP) between -38 and -59 of the core trc promoter to enhance gene expression in Ochrobactrum anthropi up to 66-fold. The high level of expression achieved by the UP element and the N-terminus fusion of a 6xHis epitope tag facilitated detection and purification of heterologous proteins directly from O. anthropi.