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Oral ingestion of a novel oxygenating compound, Ox66™, is non-toxic and has the potential to increase oxygenation
- Zhang, Fan, Aquino, Grace V., Dabi, Amjad, Nugent, William H., Song, Bjorn K., Bruce, Erica D.
- Food and chemical toxicology 2019 v.125 pp. 217-224
- digestion, digestive tract, epithelial cells, exercise, ingestion, mesentery, no observed adverse effect level, oral administration, rats, small intestine, viability assays
- Ox66™ is a novel solid state oxygenating compound. In order to support the use of Ox66™ as a potential oxygenating supplement to injured cells, this study evaluated the safety of Ox66™, its ability to withstand the conditions in the digestive tract, and its potential to increase oxygenation in the mesentery in rats. The toxicity of Ox66™ was evaluated by performing acute (10-day) and chronic (90-day) feeding studies on rats, the stability of the compound in the digestive tract was evaluated via ex vivo simulated digestion and subsequent CFDA viability assay on gut epithelial cells, and its capacity for oxygenation in the mesenteric microcirculation was determined by interstitial fluid pressure (PISF) O2 measurements upon injection into the small intestine of rats. No toxicity was found associated with acute or chronic oral administration of the compound in rats, and the compound was able to withstand the environment of the digestive tract in vitro. Based on the acute animal feeding study, the NOAEL was considered to be 1000 mg/kg/day. This proof-of-concept study further demonstrates the potential of Ox66™ to function as an oxygenating supplement that might be useful for treating either pathological hypoxic-related conditions or to improve oxygenation levels during or after exercise under healthy conditions.