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In vitro and in vivo evaluation of enzymatic and antioxidant activity, cytotoxicity and genotoxicity of curcumin-loaded solid dispersions

Author:
Silva de Sá, Igor, Peron, Ana Paula, Leimann, Fernanda Vitória, Bressan, Getúlio Nicola, Krum, Bárbara Nunes, Fachinetto, Roselei, Pinela, José, Calhelha, Ricardo Costa, Barreiro, Maria Filomena, Ferreira, Isabel C.F.R., Gonçalves, Odinei Hess, Ineu, Rafael Porto
Source:
Food and chemical toxicology 2019 v.125 pp. 29-37
ISSN:
0278-6915
Subject:
Allium cepa, Curcuma longa, acetylcholinesterase, adenocarcinoma, amine oxidase (flavin-containing), antioxidant activity, bioavailability, breasts, cell lines, cholinesterase, curcumin, cytotoxicity, dispersions, genotoxicity, glutathione transferase, hemolysis, hepatoma, in vivo studies, lungs, mutagens, nanoparticles, neoplasm cells, solubility, thiobarbituric acid-reactive substances, toxicology
Abstract:
Curcumin, the main bioactive polyphenolic compound in Curcuma longa L. rhizomes has a wide range of bioactive properties. Curcumin presents low solubility in water and thus limited bioavailability, which decreases its applicability. In this study, cytotoxic effects of curcumin solid dispersions (CurSD) were evaluated against tumor (breast adenocarcinoma and lung, cervical and hepatocellular carcinoma) and non-tumor (PLP2) cells, while cytotoxic and genotoxic effects were evaluated in Allium cepa. The effect of the CurSD on the acetylcholinesterase (AChE), butyrylcholinesterase (BChE), glutathione S-transferase (GST), and monoamine oxidase (MAO A-B) enzymes was determined, as well as its capacity to inhibit the oxidative hemolysis (OxHLIA) and the formation of thiobarbituric acid reactive substances (TBARS). CurSD are constituted by nanoparticles that are readily dispersible in water, and inhibited 24% and 64% of the AChE and BChE activity at 100 μM, respectively. GST activity was inhibited at 30 μM while MAO-A and B activity were inhibited at 100 μM. CurSD showed cytotoxicity against all the tested tumor cell lines without toxic effects for non-tumor cells. No cytotoxic and genotoxic potential was detected with the Allium cepa test. CurSD maintained the characteristics of free curcumin on the in vitro modulation of important enzymes without appreciable toxicity.
Agid:
6295821