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Anti-inflammatory effects of dicaffeoylquinic acids from Ilex kudingcha on lipopolysaccharide-treated RAW264.7 macrophages and potential mechanisms

Wan, Peng, Xie, Minhao, Chen, Guijie, Dai, Zhuqing, Hu, Bing, Zeng, Xiaoxiong, Sun, Yi
Food and chemical toxicology 2019 v.126 pp. 332-342
IKappaB kinase, Ilex kaushue, acids, anti-inflammatory activity, gene expression, inducible nitric oxide synthase, inflammation, interleukin-1beta, interleukin-6, leaves, macrophages, messenger RNA, mitogen-activated protein kinase, nitric oxide, toxicology, transcription factor NF-kappa B, tumor necrosis factor-alpha
Increasing evidence has shown that dicaffeoylquinic acids (DiCQAs) have anti-inflammatory activity. However, the underlying molecular mechanisms of the anti-inflammatory effects of DiCQAs are still unclear. In the present study, the anti-inflammatory effects of DiCQAs from the leaves of Ilex kudingcha and the potential molecular mechanisms on LPS-induced inflammatory responses in RAW264.7 macrophage cells were investigated. The results showed that pretreatment with DiCQAs could suppress the production of NO, PGE2 and also pro-inflammatory cytokines TNF-α, IL-1β and IL-6, and the mRNA expression of two major inflammatory mediators of COX-2 and iNOS. The phosphorylated IκBα, ERK, JNK and p38 proteins in LPS-treated cells were significantly increased, which could be reversed by pretreatment with DiCQAs in a concentration-dependent manner. Taken together, the results suggest that DiCQAs from I. kudingcha have potent anti-inflammatory effects on LPS-induced inflammatory responses by inhibiting the NF-κB and MAPKs pathways and may be a prophylactic for inflammation.