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Characterization of a potential ripening regulator, SlNAC3, from Solanum lycopersicum

Jing, Le, Li, Jie, Song, Yuzhu, Zhang, Jinyang, Chen, Qiang, Han, Qinqin
Open life sciences 2018 v.13 no.1 pp. 518-526
Solanum lycopersicum, Solanum pennellii, chromatin, drought, fruiting, fruits, functional properties, genome, matrix-assisted laser desorption-ionization mass spectrometry, models, polyclonal antibodies, precipitin tests, rabbits, ripening, salt stress, stress tolerance, tomatoes, transcription factors
NAC (for NAM, ATAF1-2, and CUC2) proteins are one of the largest transcription factor families in plants. They have various functions and are closely related to developmental processes of fruits. Tomato (Solanum lycopersicum) is a model plant for studies of fruit growth patterns. In this study, the functional characteristics and action mechanisms of a new NAC-type transcription factor, SlNAC3 (SGN-U568609), were examined to determine its role in tomato development and ripening. The SlNAC3 protein was produced by prokaryotic expression and used to immunize New Zealand white rabbits to obtain a specific polyclonal antibody against SlNAC3. By co-immunoprecipitation and MALDI-TOF-MS assays, we showed that there was an interaction between the SlNAC3 protein and Polygalacturonase-2 (PG-2), which is related to the ripening and softening of fruit. Chromatin immunoprecipitation assays revealed the genome of the highly stress-tolerant Solanum pennellii chromosome 10 (sequence ID, HG975449.1), further demonstrating that SlNAC3 is a negative regulator of drought and salinity stress resistance in tomato, consistent with previous reports. These results indicate that SlNAC3 is not only involved in abiotic stress, but also plays a necessary role in mediating tomato ripening.