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Molecular characterisation of a novel gemycircularvirus associated with olive trees in Italy
- Chiumenti, Michela, Greco, Claudia, Antelmi, Ilaria, Sion, Valentina, Altamura, Giuseppe, Nigro, Franco, Saldarelli, Pasquale
- Virus research 2019 v.263 pp. 169-172
- Olea europaea, branches, genome, leaves, olives, open reading frames, proteins, sequence analysis, single-stranded DNA, surveys, tissues, viruses, Italy
- A new gemycircularvirus sequence was obtained from total DNAs extracted from olive leaves and twigs tissues. Its complete genome consists of a single-stranded circular DNA of 2,145 nt, whose sequence was obtained by rolling circle amplification (RCA). Genome analysis identified three open reading frames, sharing homologies with the coat and replication-associated proteins, these latter in the anti-parallel strand, of known gemycircularvirus species. Search for homologies showed that the circular ssDNA sequence is distantly related to other gemycircularviruses thus originating from a new virus species, for which the name olive-associated gemycircularvirus 1 (OaGV1) is proposed. A survey in two different olive-growing areas of the Apulian region (Southern Italy), showed a limited distribution of OaGV1.