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A S-adenosylmethionine synthetase gene from the pathogenic piscine hemoflagellate, Cryptobia salmositica

Jesudhasan, Palmy R., Woo, Patrick T. K.
Parasitology research 2007 v.100 no.6 pp. 1401-1406
Cryptobia, DNA, Leishmania donovani, Leishmania infantum, Leishmania major, Southern blotting, Trypanosoma brucei, Trypanosoma cruzi, amino acid sequences, amino acids, fish, gene expression regulation, genes, humans, metabolites, methionine adenosyltransferase, open reading frames
We report on the identification of a Cryptobia genomic DNA gene, predict it to encode a S-adenosylmethionine synthetase signature 1 motif and propose to name it S-adenosylmethionine synthetase (MAT). The open reading frame of MAT is 1,046 bp with 341 deduced amino acids. The MAT gene was identified using universal genome walking and Southern blot analysis revealed it to be a multi-copy gene. The S-adenosylmethionine synthetase of Cryptobia salmositica amino acid sequence is similar to those of other pathogenic kinetoplastids (Leishmania donovani 71%, Leishmania major 70%, Leishmania infantum 71%, Trypanosoma brucei 72%, Trypanosoma cruzi 70% and T. cruzi strain CL Brener 70%). The C. salmositica MAT has a conserved hexapeptide GAGDQG, which is widely found in bacteria, parasitic protozoans and also in humans. These suggest that MAT may have highly conserved functions such as regulation of gene expression and biosynthesis of a multitude of essential metabolites.