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A comparative proteomic analysis of the PVY-induced hypersensitive response in leaves of potato (Solanum tuberosum L.) plants that differ in Ny-1 gene dosage

Szajko, Katarzyna, Sołtys-Kalina, Dorota, Szarzynska, Bogna, Strzelczyk-Żyta, Danuta, Szweykowska-Kulinska, Zofia, Marczewski, Waldemar
European journal of plant pathology 2019 v.153 no.2 pp. 385-396
H+/K+-exchanging ATPase, H-transporting ATP synthase, Potato virus Y, Solanum tuberosum, alleles, breeding, cultivars, gene dosage, hypersensitive response, leaves, liquid chromatography, mitochondria, monodehydroascorbate reductase (NADH), photosynthesis, potatoes, protein composition, proteins, proteomics, resistance genes, screening, tandem mass spectrometry, tetraploidy, two-dimensional gel electrophoresis
The Ny-1 gene confers hypersensitive response (HR) to Potato virus Y (PVY) in potato cultivars (Solanum tuberosum L.). Tetraploid potato breeding clone PB07–37 possessing the allele Ny-1 in a duplex state was developed. After PVY infection, the size of necrotic lesions in leaves of PB07–37 was reduced by approximately 68% in relation to cultivar Rywal plants displaying Ny-1 in a simplex dosage. Two-dimensional gel electrophoresis (2-DE) was applied for screening PVY-induced proteins in leaves of PB07–37. Compared with non-infected control plants, 60 reproducible PVY-induced proteins were detected using LC-MS/MS analysis, of which 41 were involved in qualitative changes and 19 were differently expressed in inoculated leaves by at least 1.5-fold. Proteins involved in the category of photosynthesis and primary metabolism were the most abundant. The results from PB07–37 (Ny-1 duplex) were compared with data from Rywal (Ny-1 simplex). The protein profiles in the Ny-1 simplex and Ny-1 duplex plants are genotype specific. Only eight proteins were identified in both genotypes. Five of them: ATP synthase CF1 alpha chain, chloroplastic; ATP synthase CF1 beta subunit, chloroplastic; ATP synthase subunit beta, mitochondrial-like; linoleate 13S-lipoxygenase 2–1, chloroplastic; and mitochondrial monodehydroascorbate reductase 5 are known to be involved in the defense response in plants. In our study, however, protein abundance did not correspond to the Ny-1 resistance gene dosage.