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Seedling and adult stage resistance to spot form of net blotch (SFNB) in spring barley and stability of adult stage resistance to SFNB in Morocco

Author:
Gyawali, Sanjaya, Amezrou, Reda, Verma, Ramesh Pal Singh, Brueggeman, Robert, Rehman, Sajid, Belqadi, Loubna, Arbaoui, Mustapha, Tamang, Prabin, Singh, Murari
Source:
European journal of plant pathology 2019 v.153 no.2 pp. 475-487
ISSN:
0929-1873
Subject:
Pyrenophora teres, adults, analysis of variance, disease severity, field experimentation, genotype-environment interaction, greenhouses, introgression, leaves, mature plants, net blotch, pathotypes, seedlings, spring barley, Morocco
Abstract:
The aims of this study were to identify resistance to spot form of net blotch (SFNB) in spring barley and to investigate the stability of SFNB resistance in Morocco. The seedling resistance to SFNB was evaluated by inoculating 340 barley genotypes with the Pyrenophora teres F. maculata (Ptm) isolate FGOB10Ptm-1 (FGO) in the greenhouse. The same barely genotypes were evaluated for adult-stage plant resistance to SFNB in field trials in Morocco. All experiments were conducted in alpha-lattices with two replicates. SFNB disease severity was estimated on barley leaves using double digit scale. To investigate the stability of both qualitative and quantitative resistance to SFNB, 104 barley genotypes were subjected to AMMI analysis. Differential responses of SFNB barley resistance to FGO were found at the seedling stage in the greenhouse. Twelve genotypes showing scores of <1.5, at the seedling stage, were determined to be highly resistant to FGO. The ANOVA showed highly significant (p < 0.001) effects of genotype (G) and G × E (E-Pathotypes of SFNB) interaction on SFNB severity among the 340 barley genotypes at the adult plant stage. The AMMI ANOVA showed that IPCA1, IPCA2 and IPCA3 accounted for 77.9% of the variation of the G × E interaction for SFNB severity. The G × E interaction consisted of divergent genotypic responses to SFNB severity due to different pathotypes prevalent in hot-spot environments. The AMMI stability value demonstrated that barley genotypes AM-14, 30, 31, 68, 107, 108, 112, 149, 170, 185, 204, 240, 304, 326, 326 and 337 were resistant and stable across hot-spot environments against SFNB. Divergent environmental responses of SFNB were recorded in MCH_2015, SE_2015, AT_2016, SE_2016 and JS_2015. Stable SFNB resistant genotypes are valuable resources for the introgression of qualitative and quantitative resistance to barley in Morocco.
Agid:
6298826