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Spontaneous hybridisation within Aegilops collection and biobanking of crop wild relatives (CWR)

Holubec, V., Leišová-Svobodová, L., Matějovič, M.
Genetic resources and crop evolution 2019 v.66 no.2 pp. 311-319
Aegilops crassa, Aegilops cylindrica, Aegilops geniculata, Aegilops neglecta, Aegilops triuncialis, Bayesian theory, annuals, crops, gene banks, genes, genotype, hybrids, in situ conservation, intergeneric hybridization, introgression, microsatellite repeats, outcrossing, pollinators, seed set, wheat, wild relatives
Collections of crop wild relatives (CWR) are an important part of crop genetic resources. They represent a backup of in situ conservation and provide material for use in research and breeding. They are usually maintained together with crops. Many CWRs are cross pollinators and they need special attention including isolation. Isolation decreases seed set in self-incompatible and highly outcrossing accessions. The annual Triticeae collection in the Czech Genebank was maintained in small row sown plots surrounded by several wheat rows and less related species formed larger blocks. The appeared hybrids were analysed morphologically, genetically and seed set calculated. On the molecular level, 40 microsatellite loci were used to test the samples. Data was evaluated using gene and genotype diversity indices and Bayesian approach to check the presence of genetic introgressions within tested samples. Hybrids with wheat were found mainly within accessions of Ae. triuncialis L. (19), Ae. crassa Boiss. (11), Ae. geniculata Roth (10), Ae. neglecta (Req.) ex Bertol. (9) and Ae. cylindrica Host (7). No spontaneous hybrids were noticed within other annual Triticeae genera.