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Studies on the biologically active secondary metabolites of the new spider parasitic fungus Gibellula gamsii

Kuephadungphan, Wilawan, Macabeo, Allan Patrick G., Luangsa-ard, Janet Jennifer, Tasanathai, Kanoksri, Thanakitpipattana, Donnaya, Phongpaichit, Souwalak, Yuyama, Kamila, Stadler, Marc
Mycological progress 2019 v.18 no.1-2 pp. 135-146
Araneae, Staphylococcus aureus, alkaloids, antimicrobial properties, chemical structure, fungi, high performance liquid chromatography, internal transcribed spacers, loci, mass spectrometry, new species, nuclear magnetic resonance spectroscopy, phylogeny, ribosomal DNA, secondary metabolites, spectral analysis, sporulation, Thailand
Numerous gatherings of a new species of the genus Gibellula, closely resembling the monotypic, neotropical G. mirabilis were encountered in Thailand. The taxon was cultured successfully although no in vitro sporulation was observed. The new species, Gibellula gamsii, could be distinguished from closely related other Gibellula species on the basis of morphological features and phylogenetic inferences recruiting concatenated sequences of five DNA loci including ITS, LSU, RPB1, RPB2, and EF1-α. The secondary metabolites of G. gamsii, strain BCC47868, were studied concurrently after preparative separation of the crude extract by preparative high-performance liquid chromatography (HPLC). Two new 1,3-disubstituted β-carboline alkaloids, for which we propose the trivial names, gibellamines A (1) and B (2), were isolated. The chemical structures of these compounds were elucidated by interpretation of spectral data, generated by nuclear magnetic resonance spectroscopy (NMR) and mass spectrometry (MS). The alkaloid 1 also exhibited moderate anti-biofilm activity against Staphylococcus aureus.