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Action of ultraviolet-C radiation and p-coumaric acid on stilbene accumulation and expression of stilbene biosynthesis-related genes in the grapevine Vitis amurensis Rupr.

Author:
Kiselev, Konstantin V., Ogneva, Zlata V., Suprun, Andrey R., Grigorchuk, Valeria P., Dubrovina, Alexandra S.
Source:
Acta physiologiae plantarum 2019 v.41 no.2 pp. 28
ISSN:
0137-5881
Subject:
Vitis amurensis, biosynthesis, catechol oxidase, genes, glucosyltransferases, irradiation, p-coumaric acid, peroxidase, polyphenols, resveratrol, ultraviolet radiation, viability
Abstract:
This paper investigated the effects of p-coumaric acid (CA) on stilbene biosynthesis in healthy cuttings of grapevine Vitis amurensis Rupr. under control conditions and after ultraviolet (UV-C) irradiation. It has been shown that the content of the detected six stilbenes (cis-piceid, t-piceid, t-ε-viniferin, cis-ε-viniferin, t-resveratrol, and t-δ-viniferin) increased after feeding with CA up to 0.5 mg g⁻¹ of the dry weight (DW), which is 2.4 times higher than that under control conditions. Feeding with CA and UV-C irradiation exerted a combined positive effect on the content of stilbenes increasing it to 0.7 mg g⁻¹ DW. In addition, the CA treatment improved viability of the cuttings after the UV-C treatment. The elevation in stilbene content induced by CA and UV-C correlated with induction of stilbene biosynthetic genes encoding stilbene synthases, resveratrol O-glucosyltransferase, polyphenol oxidase, and cationic peroxidase. The data indicate that feeding with the precursors of phenolic compounds could be an effective strategy for activation of stilbene production.
Agid:
6300978