Main content area

Action of ultraviolet-C radiation and p-coumaric acid on stilbene accumulation and expression of stilbene biosynthesis-related genes in the grapevine Vitis amurensis Rupr.

Kiselev, Konstantin V., Ogneva, Zlata V., Suprun, Andrey R., Grigorchuk, Valeria P., Dubrovina, Alexandra S.
Acta physiologiae plantarum 2019 v.41 no.2 pp. 28
Vitis amurensis, biosynthesis, catechol oxidase, genes, glucosyltransferases, irradiation, p-coumaric acid, peroxidase, polyphenols, resveratrol, ultraviolet radiation, viability
This paper investigated the effects of p-coumaric acid (CA) on stilbene biosynthesis in healthy cuttings of grapevine Vitis amurensis Rupr. under control conditions and after ultraviolet (UV-C) irradiation. It has been shown that the content of the detected six stilbenes (cis-piceid, t-piceid, t-ε-viniferin, cis-ε-viniferin, t-resveratrol, and t-δ-viniferin) increased after feeding with CA up to 0.5 mg g⁻¹ of the dry weight (DW), which is 2.4 times higher than that under control conditions. Feeding with CA and UV-C irradiation exerted a combined positive effect on the content of stilbenes increasing it to 0.7 mg g⁻¹ DW. In addition, the CA treatment improved viability of the cuttings after the UV-C treatment. The elevation in stilbene content induced by CA and UV-C correlated with induction of stilbene biosynthetic genes encoding stilbene synthases, resveratrol O-glucosyltransferase, polyphenol oxidase, and cationic peroxidase. The data indicate that feeding with the precursors of phenolic compounds could be an effective strategy for activation of stilbene production.