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Oxygen partial pressure plays a crucial role in B16 melanoma cell survival by regulating autophagy and mitochondrial functions

Author:
Wang, Yuqing, Wang, Yan, Wu, Jia, Wang, Wei, Zhang, Yu
Source:
Biochemical and biophysical research communications 2019 v.510 no.4 pp. 643-648
ISSN:
0006-291X
Subject:
acetylcysteine, apoptosis, autophagy, blood vessels, cell viability, hypoxia, melanoma, mitochondria, neoplasm cells, oxygen, reactive oxygen species
Abstract:
The oxygen partial pressure generally increases when cancerous cells become part of the blood vessels. The study was to investigate the influence of oxygen partial pressure on the apoptosis of B16 melanoma cells. Our results demonstrated that both short-term and long-term hypoxia/reoxygenation (H/R) treatment increased stress-induced intracellular reactive oxygen species (ROS). H/R treatment also increased apoptosis and autophagy in B16 cells. N-acetylcysteine (NAC), a ROS scavenger, can reduce ROS and aid survival. However, Bafilomycin A1, an autophagy inhibitor, can accelerate cell death. Thus, our work revealed that ROS and autophagy play critical roles in cellular H/R.
Agid:
6301079