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Study on the degradation performance and kinetics of immobilized cells in straw-alginate beads in marine environment
- Xue, Jianliang, Wu, Yanan, Shi, Ke, Xiao, Xinfeng, Gao, Yu, Li, Lin, Qiao, Yanlu
- Bioresource technology 2019 v.280 pp. 88-94
- Aneurinibacillus, Halomonas, biosurfactants, immobilized cells, marine environment, models, response surface methodology, sodium alginate, straw
- In this study, two strains Halomonas and Aneurinibacillus were mixed in equal proportions as free cells that could degrade diesel and produce biosurfactant. A new type of immobilized cells, free cells immobilized in beads combined with sodium alginate and straw, was studied. The components of straw-alginate beads were optimized by Response Surface Method, and the degradation performance of immobilized cells was determined. The result indicated that the density, strength and broken rate of straw-alginate beads were 1.04 g/cm3, 216 g and 4%, respectively. The best degradation rate of immobilized cells in straw-alginate beads could be 68.68%. Lately, by analyzing the Monod model, vmax (maximum specific degradation rate of diesel) and KS (half saturation rate constant) of immobilized cells in straw-alginate beads were 1.84 d−1 and 3.23 g/L, respectively, which explained the higher degradation performance.