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Study on the degradation performance and kinetics of immobilized cells in straw-alginate beads in marine environment

Xue, Jianliang, Wu, Yanan, Shi, Ke, Xiao, Xinfeng, Gao, Yu, Li, Lin, Qiao, Yanlu
Bioresource technology 2019 v.280 pp. 88-94
Aneurinibacillus, Halomonas, biosurfactants, immobilized cells, marine environment, models, response surface methodology, sodium alginate, straw
In this study, two strains Halomonas and Aneurinibacillus were mixed in equal proportions as free cells that could degrade diesel and produce biosurfactant. A new type of immobilized cells, free cells immobilized in beads combined with sodium alginate and straw, was studied. The components of straw-alginate beads were optimized by Response Surface Method, and the degradation performance of immobilized cells was determined. The result indicated that the density, strength and broken rate of straw-alginate beads were 1.04 g/cm3, 216 g and 4%, respectively. The best degradation rate of immobilized cells in straw-alginate beads could be 68.68%. Lately, by analyzing the Monod model, vmax (maximum specific degradation rate of diesel) and KS (half saturation rate constant) of immobilized cells in straw-alginate beads were 1.84 d−1 and 3.23 g/L, respectively, which explained the higher degradation performance.