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Membrane assisted continuous production of solvents with integrated solvent removal using liquid-liquid extraction

Author:
Survase, Shrikant A., Zebroski, Ryan, Bayuadri, Cosmas, Wang, Ziyu, Adamos, Georgios, Nagy, Grigore, Pylkkanen, Vesa
Source:
Bioresource technology 2019 v.280 pp. 378-386
ISSN:
0960-8524
Subject:
Clostridium acetobutylicum, Pinus, acetone, biomass, butanol, butyrates, ethanol, fermentation, fermenters, fractionation, genetic engineering, isopropyl alcohol, lignocellulose, liquid-liquid extraction, nutrients, solvents, sugars, wood
Abstract:
The aim of this work was to demonstrate an industrially relevant pilot scale integrated process to produce butanol and other solvents from lignocellulosic sugars produced with AVAP® biomass fractionation technology from southern pine wood. The concentrated sugars were concurrently fermented using genetically engineered Clostridium acetobutylicum to n-butanol, acetone, isopropanol and ethanol in continuous membrane assisted cell recycle fermentation with steady-state solvent productivity exceeding 10 g/L/h. The solvents from the cell free permeate were recovered with extractant of butyl butyrate in a continuous liquid-liquid extraction column and aqueous product (raffinate) along with unused sugars, nutrients, and metabolic intermediates were recycled back to the fermentors. The total solvent yield approached 0.30 g/g sugars.
Agid:
6301800