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Fuel ethanol production using xylose assimilating and high ethanol producing thermosensitive Saccharomyces cerevisiae isolated from date palm juice in Bangladesh

Talukder, Ali Azam, Adnan, Nihad, Siddiqa, Ayesha, Miah, Roni, Tuli, Jamsheda Ferdous, Khan, Shafia Tasnim, Dey, Shuvra Kanti, Lertwattanasakul, Noppon, Yamada, Mamoru
Biocatalysis and agricultural biotechnology 2019 v.18 pp. 101029
Debaryomyces hansenii, Phoenix dactylifera, Pichia kudriavzevii, Saccharomyces cerevisiae, ammonium sulfate, bioethanol, carbon, ethanol, ethanol production, genetic analysis, juices, mutants, pH, ribosomal DNA, sap, temperature, xylose, yeasts, Bangladesh
Four yeasts were isolated from Khejurer Rosh [an overnight natural fermented date palm (Phoenix dactylifera) juice/sap] at low temperature (∼5–15 °C) to produce bioethanol. Cultural, morphological, physiological, biochemical and genetic analysis were carried out under various physiological conditions. All 4-strains (Dj-1, Dj-2, Dj-3, and Dj-4) could produce bioethanol and their production rates were further investigated under various carbon sources, growth temperatures, and pHs. Among them, the highest 10% (v/v) bioethanol was estimated from the thermosensitive yeast strain Dj-3, which was grown in the medium containing 18% of total sugars and 0.05% (NH4)2SO4 at optimum temperature and pH of 25 °C and 6.0, respectively. Microscopic study and a partial 26S rDNA (D1/D2 region) sequencing identified Dj-1, and Dj-3 as Saccharomyces cerevisiae, whereas, Dj-2 and Dj-4 strains were, Pichia kudriavzevii, and Debaryomyces hansenii, respectively. The strains Dj-3 and Dj-4 could grow well in the medium containing xylose as the sole carbon source. Our results conclude that the strain Dj-3 is a natural mutant strain of Saccharomyces cerevisiae, which would be an industrially potential candidate for bioethanol production.