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A “Clickable” Photoconvertible Small Fluorescent Molecule as a Minimalist Probe for Tracking Individual Biomolecule Complexes

Author:
Jun, Joomyung V., Haney, Conor M., Karpowicz, Richard J., Giannakoulias, Sam, Lee, Virginia M.-Y., Petersson, E. James, Chenoweth, David M.
Source:
Journal of the American Chemical Society 2019 v.141 no.5 pp. 1893-1897
ISSN:
1520-5126
Subject:
cycloaddition reactions, fluorescence, fluorescent dyes, fluorescent proteins
Abstract:
Photoconvertible fluorophores can enable the visualization and tracking of a specific biomolecules, complexes, and cellular compartments with precise spatiotemporal control. The field of photoconvertible probes is dominated by fluorescent protein variants, which can introduce perturbations to the target biomolecules due to their large size. Here, we present a photoconvertible small molecule, termed CPX, that can be conjugated to any target through azide–alkyne cycloaddition (“click” reaction). To demonstrate its utility, we have applied CPX to study (1) trafficking of biologically relevant synthetic vesicles and (2) intracellular processes involved in transmission of α-synuclein (αS) pathology. Our results demonstrate that CPX can serve as a minimally perturbing probe for tracking the dynamics of biomolecules.
Agid:
6302569