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Online estimation of biomass, lipid and nitrate dynamic profile using innovative light evolution kinetic model in flat panel airlift photobioreactor for Botryococcus braunii under varying light conditions
- Khichi, Shailendra Singh, Rohith, S., Gehlot, Kartik, Dutta, B.C., Ghosh, Sanjoy
- Bioresource technology reports 2019 v.6 pp. 6-14
- Botryococcus braunii, absorbance, absorption, biomass, kinetics, light intensity, light scattering, lipid content, lipids, microalgae, models, nitrates, photobioreactors
- Light availability inside the photobioreactor is generally measured by the Lambert – Beer law. However, Lambert - Beer law assumptions are not valid at high cell density cultural conditions, because light scattering and differential absorption have significant impact on light transfer in photobioreactor. In this study Lambert – Beer law was modified by applying differential absorbance in flat panel photobioreactor. As light intensity fluctuate with location and age in the cultivation process, aconsolidated model based on Lambert – Beer law including temporal light evolution and microorganism growth is presented. The model was calibrated under specific light intensity of 133 μmol m−2 s−1 and validated using the experimental data of microalgae growth under varying light conditions of 174, 261 and 348 μmol m−2 s−1. Maximum biomass concentration, maximum lipid content and lipid productivity were 2.52 g dcw L−1, 19.76% and 114.92 mg L−1d−1 at 261 μmol m−2 s−1 light intensity respectively.