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NaCl- and cold-induced stress activate different Ca2+-permeable channels in Arabidopsis thaliana

Author:
Wang, Chao, Teng, Yibo, Zhu, Shan, Zhang, Linlin, Liu, Xunyan
Source:
Plant growth regulation 2019 v.87 no.2 pp. 217-225
ISSN:
0167-6903
Subject:
Arabidopsis thaliana, additive effect, aequorin, agricultural productivity, calcium, calcium channels, cold, cold stress, plant growth, plasma membrane, salinity, signal transduction, sodium chloride, temperature
Abstract:
Salinity (NaCl) and low temperatures significantly affect plant growth, development, and agricultural productivity. Both these stresses trigger an increase in free cytosolic Ca²⁺ concentration ([Ca²⁺]ᵢ) via Ca²⁺ influx across the plasma membrane. However, the interaction between salinity- and low temperature-induced [Ca²⁺]ᵢ increases are still poorly understood. We used aequorin to investigate the NaCl- and cold-induced [Ca²⁺]ᵢ signals in Arabidopsis thaliana. We found that the combination of NaCl and cold stimuli induced a greater increase in [Ca²⁺]ᵢ than that by either NaCl or cold separately. Therefore, we showed that these factors had an additive effect on [Ca²⁺]ᵢ increase. Following a pre-treatment of either NaCl or cold, the increase in [Ca²⁺]ᵢ significantly declined in response to a second NaCl treatment or cold exposure. After pre-treatment with NaCl, a subsequent cold stimulus suppressed [Ca²⁺]ᵢ increase to a lesser degree than that by a second NaCl treatment. A similar response was observed after pre-treatment with cold and subsequent treatment with NaCl, but the peak [Ca²⁺]ᵢ differed between them. We propose that NaCl-induced Ca²⁺ channels differ from those induced by cold and that a feedback mechanism may exist between NaCl- and cold-evoked [Ca²⁺]ᵢ. The Ca²⁺-permeable channels activated by NaCl were similar to those induced by cold stress; however, they may be expressed in different cells and activated by different signaling pathways. Our results indicated that there is a cross-adaptation in [Ca²⁺]ᵢ changes in response to NaCl- and cold-induced stresses.
Agid:
6303408