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An event of alternative splicing affects the expression of two BnCYCD3-1-like genes in Brassica napus

Guo, Yuanyuan, Li, Jie, Fang, Yan, Wan, Yunbao, Tang, Jiajia, Wei, Tao, Jiang, Xuefei, Wang, Rui, Wang, Maolin
Gene 2019 v.694 pp. 33-41
Brassica napus, abiotic stress, alternative splicing, biotic stress, cell cycle, complementary DNA, cyclins, introns, leaves, messenger RNA, roots, seeds, stems, translation (genetics)
Two full-length cDNAs of the cyclin-D3-1-like gene, named as BnCYCD3-1-like-1 and BnCYCD3-1-like-2 respectively were obtained from Brassica napus, both of which encoded a cell cycle protein CYCD3. Alternative splicing (AS) events of the two genes' transcripts were identified, assigned as BnCYCD3-1-like-1-1, BnCYCD3-1-like-1-2, BnCYCD3-1-like-2-1 and BnCYCD3-1-like-2-2 respectively. BnCYCD3-1-like-1-1 and BnCYCD3-1-like-2-1 were both fully-spliced transcripts which encoded a complete protein containing a LXCXE motif, two cyclin boxes and a PEST domain, while other two alternative splicing transcripts both resulted in the early termination of the protein translation. BnCYCD3-1-like-2-2 retained the third intron, lacking a PEST domain, while BnCYCD3-1-like-1-2 retained all the introns, lacking the C-terminal cyclin domain and a PEST domain. The expression pattern for tissue and development specification of the AS transcripts were investigated. The results showed that the standard splicing transcripts (BnCYCD3-1-like-1-1 and BnCYCD3-1-like-2-1) with complete structural domains were found with the most abundant expression in seeds, followed by leaves, and the least expression in stems. Both of BnCYCD3-1-like-2-1 and BnCYCD3-1-like-2-2 had the highest abundance in leaves, followed by roots. In addition, by applying various biotic and abiotic stresses on Brassica napus, the variations in the expression of each transcript under stress treatment were studied. Also, it was found that AS of the cyclin-D3-1-like gene may play an important role in helping Brassica napus respond to environmental stresses by coordinating the levels of transcripts of standard splicing and alternative splicing.