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Genome-wide identification, characterization and expression analysis of NF-Y gene family in relation to fruit ripening in banana

Yan, Huiling, Wu, Fuwang, Jiang, Guoxiang, Xiao, Lu, Li, Zhiwei, Duan, Xuewu, Jiang, Yueming
Postharvest biology and technology 2019 v.151 pp. 98-110
1-methylcyclopropene, bananas, chromosomes, ethylene, genes, phylogeny, plant growth, regulatory sequences, ripening, stress response, transactivators
Nuclear factor Y (NF-Y) is a heterotrimeric transcription factor, which plays important roles in plant growth, development and stress responses. Yet little information regarding the role of NF-Y in regulating fruit ripening is available. In this study, a total of 44 NF-Y genes (14 MaNF-YAs, 16 MaNF-YBs, and 14 MaNF-YCs) were isolated and confirmed from the banana genome. Their distribution on chromosomes, gene structures, phylogenetic relationship with other species, cis-acting elements, genome synteny and tissue-specific expression were characterized. Furthermore, it was found that expression of six MaNF-Ys, i.e. MaNF-YA5/B1/B2/C9/C11/C14, were up-regulated as harvested fruit ripened, and their expression were accelerated by ethylene, but delayed by 1-MCP. In contrast, harvested banana fruit ripening was accompanied by down-regulation of MaNF-YA1/A3/A6/B3/B6/C2/C5. Ethylene accelerated their down-regulation while 1-MCP delayed the process. These results indicated that MaNF-YA5/B1/B2/C9/C11/C14 and MaNF-YA1/A3/A6/B3/B6/C2/C5 were implicated in the regulation of harvested banana fruit ripening, functioning as transcriptional activators and repressors, respectively.