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Use of simple sequence repeat markers for DNA fingerprinting and diversity analysis of sugarcane (Saccharum spp) cultivars resistant and susceptible to red rot

Author:
Hameed, U., Pan, Y.-B., Muhammad, K., Afghan, S., Iqbal, J.
Source:
Genetics and molecular research 2012 v.11 no.2 pp. 1195
ISSN:
1676-5680
Subject:
DNA fingerprinting, Glomerella tucumanensis, Saccharum, alleles, cultivar identification, cultivars, disease resistance, fungal diseases of plants, fungi, genetic markers, genetic polymorphism, genetic relationships, genetic variation, microsatellite repeats, sugarcane, trees, Pakistan
Abstract:
Red rod is an economically important disease of sugarcane caused by the fungus Colletotrichum falcatum. We used a simple sequence repeat (SSR)-based marker system to identify and analyze genetic relationships of red rot resistant and susceptible sugarcane cultivars grown in Pakistan. Twenty-one highly polymorphic SSR markers were used for DNA fingerprinting and genetic diversity analysis of 20 sugarcane cultivars. These SSR markers were found to be highly robust; we identified 144 alleles, with 3-11 alleles per marker and a mean of 6.8. Three SSR markers were able to identify all 20 cultivars. DNAMANĀ®-generated homology tree was used to analyze genetic diversity among these cultivars; all cultivars shared 58% or more similarity. We correlated polymorphism information content and resolving power values with marker effectiveness in the process of sugarcane cultivar identification. We concluded that a small number of SSR-derived DNA markers will allow breeders to identify red rot resistant and susceptible cultivars.
Agid:
63043
Handle:
10113/63043