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The Ep152R ORF of African swine fever virus strain Georgia encodes for an essential gene that interacts with host protein BAG6

Author:
Borca, Manuel V., O’Donnell, Vivian, Holinka, Lauren G., Rai, Devendra K., Sanford, Brenton, Alfano, Marialexia, Carlson, Jolene, Azzinaro, Paul A., Alonso, Covadonga, Gladue, Douglas P.
Source:
Virus research 2016 v.223 pp. 181-189
ISSN:
0168-1702
Subject:
African swine fever virus, Vaccinia virus, etiological agents, fluorescence microscopy, genes, immune response, open reading frames, swine, two hybrid system techniques, viral proteins, virus replication, viruses
Abstract:
African swine fever virus (ASFV) is the etiological agent of a contagious and often lethal disease of domes-tic pigs that has significant economic consequences for the swine industry. The viral genome encodes for more than 150 genes, and only a select few of these genes have been studied in some detail. Here we report the characterization of open reading frame Ep152R that has a predicted complement control module/SCR domain. This domain is found in Vaccinia virus proteins that are involved in blocking the immune response during viral infection. A recombinant ASFV harboring a HA tagged version of the Ep152R protein was developed (ASFV-G-Ep152R-HA) and used to demonstrate that Ep152R is an early virus protein.Attempts to construct recombinant viruses having a deleted Ep152R gene were consistently unsuccessful indicating that Ep152R is an essential gene. Interestingly, analysis of host-protein interactions for Ep152Rusing a yeast two-hybrid screen, identified BAG6, a protein previously identified as being required for ASFV replication. Furthermore, fluorescent microscopy analysis confirms that Ep152R-BAG6 interaction actually occurs in cells infected with ASFV.
Agid:
63049
Handle:
10113/63049