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Induced systemic resistance against Cucumber mosaic virus by Phoma sp. GS8‐2 stimulates transcription of pathogenesis‐related genes in Arabidopsis

Author:
Elsharkawy, Mohsen Mohamed
Source:
Pest management science 2019 v.75 no.3 pp. 859-866
ISSN:
1526-498X
Subject:
Arabidopsis, Cucumber mosaic virus, Phoma, barley, chitinase, cucumbers, enzyme-linked immunosorbent assay, filtrates, gene expression regulation, genes, inoculum, pathogenesis, pest management, quantitative polymerase chain reaction, reverse transcriptase polymerase chain reaction, systemic acquired resistance, transcription (genetics), vegetable growing
Abstract:
BACKGROUND: Cucumber mosaic virus (CMV) is a serious threat to vegetable production worldwide. The efficacy of Phoma sp. GS8‐2 was evaluated against CMV in Arabidopsis and cucumber plants. RESULTS: Arabidopsis and cucumber plants treated with barley grain inoculum (BGI) or cell‐free filtrate (CF) of GS8‐2 demonstrated decreased CMV severity and titre using enzyme‐linked immunosorbent assay relative to the control. Cucumber growth and yield parameters were significantly increased due to colonization with GS8‐2 under field conditions. Molecular mechanisms underlying mediated resistance induced by GS8‐2 against CMV were investigated. Real‐time polymerase chain reaction (RT‐PCR) results confirmed that both BGI and CF of GS8‐2 stimulated the transcription levels of pathogenesis related genes (β1‐3 glucanase, chitinase, PR1, PAL1 and LOX1), which could be involved in induced resistance against CMV. CONCLUSION: Exploring the expression of the highly upregulated genes in GS8‐2‐induced plants suggested the contribution of multiple plant defence pathways against CMV. © 2018 Society of Chemical Industry
Agid:
6305478