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Antifouling Microparticles To Scavenge Lipopolysaccharide from Human Blood Plasma

Vorobii, Mariia, Kostina, Nina Yu., Rahimi, Khosrow, Grama, Silvia, Söder, Dominik, Pop-Georgievski, Ognen, Sturcova, Adriana, Horak, Daniel, Grottke, Oliver, Singh, Smriti, Rodriguez-Emmenegger, Cesar
Biomacromolecules 2019 v.20 no.2 pp. 959-968
adsorption, antifouling activities, blood plasma, catalysts, coagulation, copper, endotoxins, humans, lipopolysaccharides, microparticles, polymerization, polymyxin B, porosity, proteins, sepsis (infection)
Currently, one of the most promising treatments of lipopolysaccharides (LPS)-induced sepsis is based on hemofiltration. Nevertheless, proteins rapidly adsorbed on the artificial surface of membranes which leads to activation of coagulation impairing effective scavenging of the endotoxins. To overcome this challenge, we designed polymer-brush-coated microparticles displaying antifouling properties and functionalized them with polymyxin B (PMB) to specifically scavenge LPS the most common endotoxin. Poly[(N-(2-hydroxypropyl) methacrylamide)-co-(carboxybetaine methacrylamide)] brushes were grafted from poly(glycidyl methacrylate) microparticles using photoinduced single-electron transfer living radical polymerization (SET-LRP). Notably, only parts-per-million of copper catalyst were necessary to achieve brushes able to repel adsorption of proteins from blood plasma. The open porosity of the particles, accessible to polymerization, enabled us to immobilize sufficient PMB to selectively scavenge LPS from blood plasma.