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Quantitative imaging of 33P in plant materials using 14C polymer references
- Koch, Maximilian, Schiedung, Henning, Siebers, Nina, McGovern, Sean, Hofmann, Diana, Vereecken, Harry, Amelung, Wulf
- Analytical and bioanalytical chemistry 2019 v.411 no.6 pp. 1253-1260
- autoradiography, carbon, corn, cost effectiveness, image analysis, leaves, luminescence, phosphorus, plant tissues, polymers, radionuclides, sediments, soil, wheat
- Phosphorus (P) research still lacks techniques for rapid imaging of P use and allocation in different soil, sediment, and biological systems in a quantitative manner. In this study, we describe a time-saving and cost-efficient digital autoradiographic method for in situ quantitative imaging of ³³P radioisotopes in plant materials. Our method combines autoradiography of the radiotracer applications with additions of commercially available ¹⁴C polymer references to obtain ³³P activities in a quantitative manner up to 2000 Bq cm⁻². Our data show that linear standard regressions for both radioisotopes are obtained, allowing the establishment of photostimulated luminescence equivalence between both radioisotopes with a factor of 9.73. Validating experiments revealed a good agreement between the calculated and applied ³³P activity (R² = 0.96). This finding was also valid for the co-exposure of ¹⁴C polymer references and ³³P radioisotope specific activities in excised plant leaves for both maize (R² = 0.99) and wheat (R² = 0.99). The outlined autoradiographic quantification procedure retrieved 100% ± 12% of the ³³P activity in the plant leaves, irrespective of plant tissue density. The simplicity of this methodology opens up new perspectives for fast quantitative imaging of ³³P in biological systems and likely, thus, also for other environmental compartments.