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Effects of yeast culture supplementation and the ratio of non-structural carbohydrate to fat on rumen fermentation parameters and bacterial-community composition in sheep

Author:
Liu, Yang-zhi, Chen, Xue, Zhao, Wei, Lang, Min, Zhang, Xue-feng, Wang, Tao, Farouk, Mohammed Hamdy, Zhen, Yu-guo, Qin, Gui-xin
Source:
Animal feed science and technology 2019
ISSN:
0377-8401
Subject:
Olsenella, Ruminococcus, acetic acid, ammonium nitrogen, butyric acid, cannulas, carbohydrates, diet, liquids, pH, rumen fermentation, sheep, volatile fatty acids, yeasts
Abstract:
This study aimed to evaluate the effects of yeast culture (YC) supplementation and the ratio of non-structural carbohydrate to fat (NSCFR) on rumen fermentation parameters (RFPs) and bacterial-community composition (BCC) using 6 ruminally cannulated sheep in a 2 × 3 factorial experiment. We formulated 6 dietary treatments with 3 levels of YC supplementation (0, 0.8 and 2.3 g/kg of dietary dry matter) and 2 dietary NSCFRs (17.02 and 5.60). Each animal was fed 1 of 6 diets in a 6 × 6 Latin square arrangement during the experiment. Ruminal liquid was sampled at 0, 2, 4, 6, 8, and 12 hours after morning feeding on days 16 and 17 (last 2 days) in all 6 of the Latin square periods. The concentrations of acetic acid, butyric acid and total volatile fatty acid (TVFA) were significantly (p < 0.05) increased and the pH values were significantly (p < 0.05) decreased when the animals fed on high-NSCFR diet (H diet) with 2.3 g/kg of YC at 2 hour after feeding compared with that on the low-NSCFR diet (L diet). However, the concentrations of ammonia nitrogen (NH3-N) were higher (p < 0.05) in all H groups than that in L groups. The relative abundances of Prevotella_1 were higher in all L groups (p < 0.05), while Lachnospiraceae_NK3A20 and Olsenella were higher in H groups (p < 0.05). Compared with L groups, the relative abundances of Candidatus_Saccharimonas and [Ruminococcus] gauvreauii of H groups were increased when supplemented with YC (2.3 g/kg; p < 0.05). A significantly increased relative abundance of Butyrivibrio_2 was observed in the L group (2.3 g/kg YC). Overall, the RFPs and BCC were influenced by both dietary NSCFR and YC supplementation. There was significant interaction between dietary NSCFR and YC supplementation in the aspects of VFAs, NH3-N and all genera of BCC.
Agid:
6307207