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Effect of ginsenoside Rh2 on renal apoptosis in cisplatin-induced nephrotoxicity in vivo

Qi, Zeng, Li, Wei, Tan, Jing, Wang, Cuizhu, Lin, Hongqiang, Zhou, Baisong, Liu, Jinping, Li, Pingya
Phytomedicine 2019 v.61 pp. 152862
Panax ginseng, animal models, apoptosis, blood serum, caspase-3, caspase-8, caspase-9, cisplatin, cytochrome c, histopathology, inflammation, ingredients, intraperitoneal injection, kidneys, metabolites, nephrotoxicity, oxidative stress, therapeutics, tissues
Ginsenoside Rh2 (Rh2), an important ingredient from Panax ginseng, has received much attention due to a range of pharmacological actions.The aim of the study was to investigate the therapeutic potential Rh2 on cisplatin (CDDP)-induced nephrotoxicity and to elucidate involved mechanisms.An in vivo mice model of CDDP-induced nephrotoxicity was established by a single intraperitoneal injection of CDDP (20 mg/kg) to assess the effects of Rh2 on renal biochemical parameter, oxidative stress, inflammation tubular cell apoptosis and serum metabolic profiles.Rh2 protected against CDDP-induced renal dysfunction and ameliorated CDDP-induced oxidative stress, histopathological damage, inflammation and tubular cell apoptosis in kidney. Rh2 treatment had significantly increased expression of Bcl-2 and decreased expression of p53, Bax, cytochrome c, caspase-8, caspase-9, and caspase-3 in kidney tissues. Metabolomic analysis identified 29 altered serum metabolites in Rh2 treatment mice.These results suggest that Rh2 protects against CDDP-induced nephrotoxicity via action on caspase-mediated pathway.