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Effect of ginsenoside Rh2 on renal apoptosis in cisplatin-induced nephrotoxicity in vivo

Author:
Qi, Zeng, Li, Wei, Tan, Jing, Wang, Cuizhu, Lin, Hongqiang, Zhou, Baisong, Liu, Jinping, Li, Pingya
Source:
Phytomedicine 2019 v.61 pp. 152862
ISSN:
0944-7113
Subject:
Panax ginseng, animal models, apoptosis, blood serum, caspase-3, caspase-8, caspase-9, cisplatin, cytochrome c, histopathology, inflammation, ingredients, intraperitoneal injection, kidneys, metabolites, nephrotoxicity, oxidative stress, therapeutics, tissues
Abstract:
Ginsenoside Rh2 (Rh2), an important ingredient from Panax ginseng, has received much attention due to a range of pharmacological actions.The aim of the study was to investigate the therapeutic potential Rh2 on cisplatin (CDDP)-induced nephrotoxicity and to elucidate involved mechanisms.An in vivo mice model of CDDP-induced nephrotoxicity was established by a single intraperitoneal injection of CDDP (20 mg/kg) to assess the effects of Rh2 on renal biochemical parameter, oxidative stress, inflammation tubular cell apoptosis and serum metabolic profiles.Rh2 protected against CDDP-induced renal dysfunction and ameliorated CDDP-induced oxidative stress, histopathological damage, inflammation and tubular cell apoptosis in kidney. Rh2 treatment had significantly increased expression of Bcl-2 and decreased expression of p53, Bax, cytochrome c, caspase-8, caspase-9, and caspase-3 in kidney tissues. Metabolomic analysis identified 29 altered serum metabolites in Rh2 treatment mice.These results suggest that Rh2 protects against CDDP-induced nephrotoxicity via action on caspase-mediated pathway.
Agid:
6307912