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Enhancement and modeling of enzymatic hydrolysis on cellulose from Agave bagasse hydrothermally pretreated in a horizontal bioreactor
- Pino, Marcela Sofía, Rodríguez-Jasso, Rosa M., Michelin, Michele, Ruiz, Héctor A.
- Carbohydrate polymers 2019
- Agave, X-ray diffraction, bagasse, biofuels, biomass, bioreactors, cellulose, cost effectiveness, endo-1,4-beta-glucanase, enzymatic hydrolysis, fuel production, glucose, lignocellulose, mathematical models, mixing, saccharification, scanning electron microscopy
- One of the major challenges in biofuels production from lignocellulosic biomass is the generation of high glucose titers from cellulose in the enzymatic hydrolysis stage of pretreated biomass to guarantee a cost-effective process. Therefore, the enzymatic saccharification on cellulose at high solid loading is an alternative. In this work, the agave bagasse was hydrothermally pretreated and optimized at 194°C/30min, obtaining a pretreated solid rich in cellulose content (>46.46%), and subjected to enzymatic hydrolysis at high solid levels. A horizontal bioreactor was designed for enzyme saccharification at high solid loadings [25% (w/v)]. The bioreactor improved mixing efficiency, with cellulose conversions up to 98% (195.6g/L at 72h). Moreover, mathematical modeling of cellulase deactivation demonstrated that cellulases lose most of their initial activity in the first hours of the reaction. Also, cellulose was characterized by X-ray diffraction, and the pretreated solids were visualized using scanning electron microscopy.