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Scandenolone from Cudrania tricuspidata fruit extract suppresses the viability of breast cancer cells (MCF-7) in vitro and in vivo

Jiang, Xinwei, Cao, Chunting, Sun, Weiwei, Chen, Zisheng, Li, Xusheng, Nahar, Lutfun, Sarker, Satyajit D., Georgiev, Milen I., Bai, Weibin
Food and chemical toxicology 2019 v.126 pp. 56-66
Maclura tricuspidata, animal models, apoptosis, breast neoplasms, breasts, caspase-3, epithelial cells, fruit extracts, fruits, gene expression regulation, hepatocytes, humans, isoflavones, membrane potential, mitochondrial membrane, mitogen-activated protein kinase, mitosis, neoplasm cells, phosphorylation, toxicity, toxicology, viability
Scandenolone, an isoflavone, has shown anti-cancer potential. In this study, we extracted scandenolone from Cudrania tricuspidata fruit and evaluated its anti-breast cancer effects as well as toxicity in cell and animal models. In cell model, scandenolone suppressed the breast cancer MCF-7 cells viability, ceased mitotic cell cycle, decreased mitochondrial membrane potential, up-regulated cleaved caspase-3 and promoted the phosphorylation of p53. Additionally, this isoflavone promoted cell apoptosis and induced a sustained activation of the phosphorylation of p38 and ERK, but not JNK and Akt. The effects were further verified in a human MCF-7 breast cancer xenograft model, where scandenolone efficiently suppressed the cancer growth and increased apoptotic cells in tumor tissue. However scandenolone has also shown certain toxicity to normal hepatocytes and breast epithelial cells. It could be concluded that scandenolone suppressed the growth of breast cancer cells, but its toxicity towards normal cells might limit its potential clinical use.