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“Top” or “bottom” switches of a cyclohexanone monooxygenase controlling the enantioselectivity of the sandwiched substrate

Author:
Hu, Yujing, Wang, Jie, Cen, Yixin, Zheng, He, Huang, Meilan, Lin, Xianfu, Wu, Qi
Source:
Chemical communications 2019 v.55 no.15 pp. 2198-2201
ISSN:
1364-548X
Subject:
cyclohexanones, enantioselectivity, enzymes, lactones, mutation, oxidation
Abstract:
Single mutation at the switch residues F432 (F432I/L) or L435 (L435A/G) efficiently reversed the inherent enantiopreference of WT CHMOAcᵢₙₑₜₒ in the Baeyer–Villiger oxidation of various 4-phenyl-cyclohexanone derivatives and 4-alkyl-cyclohexanones, producing a series of substituted lactones with inversed configuration (up to 99% ee and 99% conversion).
Agid:
6310371