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Quantification of azide groups on a material surface and a biomolecule using a clickable and cleavable fluorescent compound
- Sakai, Rika, Iguchi, Hiroki, Maruyama, Tatsuo
- RSC advances 2019 v.9 no.8 pp. 4621-4625
- azides, fluorescent dyes, solvents
- Here, we propose a novel method for quantifying azide groups on a solid surface and a protein using a clickable and cleavable fluorescent compound. The clickable and cleavable fluorescent compound conjugates with the azide groups on the material surface and the fluorophore is then liberated into the solvent via a cleavage reaction, which can be simply quantified with a conventional fluorometer.