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The use of tissue culture and in-vitro approaches for the study of tree diseases

Fenning, Trevor M.
Plant cell, tissue, and organ culture 2019 v.136 no.3 pp. 415-430
bacteria, crops, fungi, insects, pests, plant tissues, tissue culture, tree diseases, trees
This article aims to review and discuss how to most effectively use tissue culture approaches as an aid to the study of tree diseases and pest syndromes. Firstly, the existing scientific literature is reviewed for how plant tissue culture techniques have been used to study various tree diseases in the past, with some reference to similar work that has been undertaken with other plants where relevant. In particular, the difficulties and limitations of trying to screen for resistant plants by exposing tree tissue cultures directly to disease causing organisms or extracts of them (mainly fungi, but also bacteria and even insects) is discussed at length. Examples are then provided for how even basic tissue culture procedures can greatly aid the study of tree disease processes, mainly by helping to organise and produce the plant material needed for such work according to need and at any time of year. This is especially important when working with trees, because they are much more difficult to study than short-lived crop plants, and the integration of tissue culture approaches into this work is an essential tool in this endeavour.