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Isolation of two novel promoters from ramie (Boehmeria nivea L. Gaudich) and its functional characterization in Arabidopsis thaliana

Guo, Pingan, Zheng, Yancheng, Chen, Jie, Wang, Bo, Liu, Lijun, Feng, Enying, Peng, Dingxiang
Plant cell, tissue, and organ culture 2019 v.136 no.3 pp. 467-478
Arabidopsis thaliana, Boehmeria nivea, apical meristems, copper sulfate, flowers, fruits, genetic engineering, genetic transformation, promoter regions, ramie, seedlings, shoot meristems
The development of tissue-specific or inducible promoters is important for plant genetic engineering. In this study, we isolated two novel promoters (named BnGDSₚᵣₒ and BnSTMₚᵣₒ) from ramie and analyzed their functions in Arabidopsis. The results show that BnGDSₚᵣₒ and its 5′-truncated fragments drive GUS dominantly expressed in shoot meristem while BnSTMₚᵣₒ and its 5′-truncated fragments have apical meristem specificity in Arabidopsis seedlings and nodule specificity in the flowers and siliques. The − 1233 to − 914 region and − 425 to 0 region of BnSTMₚᵣₒ are responsible for apical root specificity and root-knot specificity, respectively. In particular, CuSO₄ treatment reveals that both BnGDSₚᵣₒ and BnSTMₚᵣₒ are copper-inducible promoters. Our findings suggest that BnGDSₚᵣₒ and BnSTMₚᵣₒ and their truncated fragments have potential applications in plant genetic transformation.