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The preparation and therapeutic roles of scFv-Fc antibody against Staphylococcus aureus infection to control bovine mastitis

Author:
Wang, Man, Wang, Tingting, Guan, Yu, Wang, Fengqing, Zhu, Jianguo
Source:
Applied microbiology and biotechnology 2019 v.103 no.4 pp. 1703-1712
ISSN:
0175-7598
Subject:
Escherichia coli, Staphylococcus aureus, Western blotting, antibodies, antigens, bacterial growth, bacteriophages, bovine mastitis, cattle, culture media, dairy industry, dose response, horseradish, immunoglobulin G, neutrophils, phagocytosis, therapeutics, vaccines
Abstract:
Staphylococcus aureus–induced bovine mastitis causes significant losses to the dairy industry and available vaccines do not confer adequate protection. As a more attractive alternative, we propose the use of antibody (Ab) therapy. In our previous study, we constructed a bovine single-chain variable fragment (scFv) Ab phage display and successfully obtained scFvs that bound to S. aureus antigens with high affinity. Here, we describe a novel Ab against S. aureus (scFv-Fc Ab). To construct the scFv-Fc Ab, the scFv Ab was genetically fused to the Fc fragment of a bovine IgG1 Ab. Western blot analysis showed that the bovine scFvs-Fc Abs were successfully expressed with horseradish peroxidase–conjugated goat-anti-bovine IgG (Fc) Ab in Escherichia coli cells. The purified bovine scFvs-Fc Abs had good binding activity to S. aureus and effectively inhibited the bacterial growth in culture medium and bovine scFvs-Fc Abs enhanced phagocytosis of S. aureus by neutrophils isolated from peripheral blood in a dose-dependent manner. In the experiment of bovine scFvs-Fc Abs for the treatment of S. aureus–induced bovine mastitis, the total effective percentage reached 82% (9/11). These novel bovine scFvs-Fc Abs may be useful as therapeutic candidates for the prevention and treatment of S. aureus–induced bovine mastitis.
Agid:
6313592