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Disomic chromosome addition from Thinopyrum intermedium to bread wheat appears to confer stripe rust resistance

Author:
Nie, Linman, Yang, Yuening, Zhang, Jun, Fu, Tihua
Source:
Euphytica 2019 v.215 no.3 pp. 56
ISSN:
0014-2336
Subject:
Thinopyrum intermedium, Triticum aestivum, chromosome addition, chromosome translocation, chromosomes, disease resistance, fluorescence in situ hybridization, genes, genetic markers, genomics, grain yield, hybrids, intergeneric hybridization, meiosis, plant breeding, polymerase chain reaction, seeds, spikelets, stripe rust, wheat
Abstract:
A wheat line 14-569 developed from a hybrid of common wheat cv. Chuannong 10 with the wheat–Thinopyrum intermedium partial amphiploid TAI7045 exhibits stable double spikelets per node character in the lower middle portion of a spike, is highly resistant to stripe rust, and produces kernels with a higher 1000-kernel weight than those of its wheat parent. Chromosome counting and an analysis of meiosis demonstrated that line 14-569 is a disomic addition line with 2n = 44, 22II. Sequential genomic in situ hybridization and non-denaturing fluorescence in situ hybridization analyses indicated that one pair of St-genomic chromosomes from Th. intermedium was added to the wheat complement and that the wheat complement included one pair of 1RS/1BL wheat–rye translocation chromosomes. PCR-based landmark unique gene molecular marker analysis revealed that the added pair of chromosomes comprised 3St chromosomes, which suggested that the added 3St chromosomes from Th. intermedium might carry the genes to control the high yield-related characters and stripe rust resistance. This addition line will potentially be useful resource for improvements in wheat yields and stripe rust resistance.
Agid:
6314073