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Rapid quantification of cellulose nanocrystals by Calcofluor White fluorescence staining
- Peretz, Roi, Mamane, Hadas, Sterenzon, Elizaveta, Gerchman, Yoram
- Cellulose 2019 v.26 no.2 pp. 971-977
- acid hydrolysis, biomass, cellulose, fluorescence, fluorescent dyes, nanocrystals, pH, rapid methods, staining
- Cellulose nanocrystals (CNCs) have gained increased interest worldwide for their unique properties. CNC production by acid hydrolysis of cellulose-rich biomass is well established, but its' quantification is still complicated. In this study, a rapid method for the determination of CNC concentration using Calcofluor White (CW) fluorescence dye is demonstrated for both purified and homemade CNCs recovered from Whatman filter paper. The method is robust, selective for crystalline cellulose, suitable for routine measurement in CNC production and that the pH must be basic for staining using CW.