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Lactic acid bacteria isolated from fermented flour of finger millet, its probiotic attributes and bioactive properties

Divisekera, Divisekera Mudiyanselage Wasundara Devanmini, Samarasekera, Jayanetti Koralalage Ramani Radhika, Hettiarachchi, Chamari, Gooneratne, Jaanaki, Choudhary, Muhammad Iqbal, Gopalakrishnan, Subramaniam, Wahab, Atia-tul
Annals of microbiology 2019 v.69 no.2 pp. 79-92
Bacillus cereus, Brevibacillus borstelensis, Eleusine coracana, Enterococcus faecium, Lactobacillus delbrueckii subsp. lactis, Lactobacillus fermentum, Lactobacillus plantarum, Lactococcus lactis, Lactococcus plantarum, Paenibacillus, Pediococcus acidilactici, Streptococcus lutetiensis, adenocarcinoma, adhesion, antibacterial properties, antibiotics, antioxidant activity, bile, cell lines, cholesterol, colon, colorectal neoplasms, free radical scavengers, gastric juice, hydrophobicity, inhibitory concentration 50, lactic acid bacteria, millet flour, multiple drug resistance, neoplasm cells, pathogens, phenol, probiotics, ribosomal RNA, sequence analysis, temperature, Sri Lanka
This study aims to isolate and identify lactic acid bacteria from fermented flour of selected finger millet varieties grown in Sri Lanka and to evaluate their probiotic attributes and bioactive properties in vitro. Fifteen lactic acid bacteria were isolated from three varieties of fermented finger millet flour namely ravi, raavana and oshadha. These isolates were screened for phenotypical and biochemical characteristics. The selected isolates were identified by 16 S rRNA sequencing as Bacillus cereus (five strains), Streptococcus lutetiensis, Lactobacillus plantarum, Lactobacillus fermentum (two strains), Brevibacillus borstelensis, Paenibacillus species, Lactococcus lactis subspecies lactis, Enterococcus faecium, Pediococcus acidilactici, and Enterococcus lactis, and their partial sequences were deposited in GenBank. Among them, five isolates including two isolates, L. plantarum MF405176.1 and L. fermentum MF033346.1 isolated from ravi; two isolates, L. lactis MF480428.1 and E. faecium MF480431.1 isolated from raavana; and P. acidilactici MF480434.1 isolated from oshadha varieties respectively, exhibited in vitro safety attributes and could tolerate acid, gastric juice, bile, salt, phenol, and temperature under simulated gastric conditions, and also were susceptible to antibiotics tested. Further, they demonstrated bactericidal activity against both drug-sensitive and multidrug-resistant pathogens. Among the selected isolates, L. plantarum MF405176.1 demonstrated highest hydrophobicity and adhesion to both colon colorectal adenocarcinoma and colon colorectal carcinoma cell lines. L. lactis subspecies lactis MF480428.1 exhibited the highest auto-aggregation and 2, 2, diphenyl-1-pricrylhydrazyl free radical scavenging activity. P. acidilactici MF480434.1 demonstrated the lowest IC₅₀ values against HCT-116 and HT-29 cells. None of the LAB isolates could assimilate > 10% cholesterol in vitro.