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Genome sequence analysis of the Indian strain Mannheimia haemolytica serotype A2 from ovine pneumonic pasteurellosis

Sahay, Swati, Shome, Rajeswari, Sankarasubramanian, Jagadesan, Vishnu, Udayakumar S., Prajapati, Awadhesh, Natesan, Krithiga, Shome, Bibek Ranjan, Rahman, Habibur, Rajendhran, Jeyaprakash
Annals of microbiology 2019 v.69 no.2 pp. 151-160
Mannheimia haemolytica, bacteria, calves, gene pool, genomic islands, genomics, hosts, multiple drug resistance, pathogenesis, pathogens, phylogeny, pneumonic pasteurellosis, polymerase chain reaction, resistance genes, sequence analysis, serotypes, sheep, virulence, India
Mannheimia haemolytica is a leading causative agent of pasteurellosis in ruminants. Genome of M. haemolytica strains from different hosts has been sequenced worldwide to understand its pathogenesis. There are only few reports on the isolation of M. haemolytica in India with limited information on its molecular characteristics. The present study focuses on genome sequence analysis of a M. haemolytica strain isolated from pneumonic sheep. Mannheimia haemolytica A2 strain NIVEDI/MH/1 was isolated and identified by species and serotype-specific PCRs. Whole genome sequencing was performed using the Ion Torrent Personal Genome Machine. A comparative genomic analysis was performed to understand the virulence determinants of the Indian strain and its phylogenetic relationship with other global strains. Sequence data revealed a draft genome of 2,211,426 bp size with 41.3% GC content, assembled into 17 contigs, and contained 2379 genes. Five genomic islands identified in the genome showed high sequence identity with other respiratory pathogens of the Pasteurellaceae family. Phylogenetic analysis showed M. haemolytica A2 NIVEDI/MH/1 is very close to a M. haemolytica A2 strain from pneumonic calf. Further, the analysis revealed the presence of virulence, metal-, and multidrug resistance genes needed for pathogenesis and survival of the bacteria during infection. Also, we identified the presence of type I-C and type II-C of CRISPR-Cas arrays in the present sequenced genome. The study emphasizes the role of M. haemolytica in respiratory infections of ruminants in the Indian subcontinent and indicates the role of vertical and horizontal gene pools in pathogenicity and survivability of the bacteria.