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Function and Distribution of a Lantipeptide in Strawberry Fusarium Wilt Disease–Suppressive Soils

Author:
Kim, Da-Ran, Jeon, Chang-Wook, Shin, Jae-Ho, Weller, David M., Thomashow, Linda, Kwak, Youn-Sig
Source:
Molecular plant-microbe interactions 2019 v.32 no.3 pp. 306-312
ISSN:
0894-0282
Subject:
Fusarium oxysporum, Fusarium wilt, Streptomyces griseus, antibacterial properties, heat stability, reverse transcriptase polymerase chain reaction, rhizosphere, secondary metabolites, soil, strawberries
Abstract:
Streptomyces griseus S4-7 is representative of strains responsible for the specific soil suppressiveness of Fusarium wilt of strawberry caused by Fusarium oxysporum f. sp. fragariae. Members of the genus Streptomyces secrete diverse secondary metabolites including lantipeptides, heat-stable lanthionine-containing compounds that can exhibit antibiotic activity. In this study, a class II lantipeptide provisionally named grisin, of previously unknown biological function, was shown to inhibit F. oxysporum. The inhibitory activity of grisin distinguishes it from other class II lantipeptides from Streptomyces spp. Results of quantitative reverse transcription-polymerase chain reaction with lanM-specific primers showed that the density of grisin-producing Streptomyces spp. in the rhizosphere of strawberry was positively correlated with the number of years of monoculture and a minimum of seven years was required for development of specific soil suppressiveness to Fusarium wilt disease. We suggest that lanM can be used as a diagnostic marker of whether a soil is conducive or suppressive to the disease.
Agid:
6315883