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Ultrasound-enhanced scintillation proximity assay for rapid diagnostics

Author:
Lee, So-Young, Lim, Jae-Cheong, Cho, Eun-Ha, Lee, Seung-Kon, Jung, Sung-Hee
Source:
Analytical biochemistry 2019 v.570 pp. 51-55
ISSN:
0003-2697
Subject:
antibodies, antigens, carcinogenesis, coatings, colorectal neoplasms, diagnostic techniques, humans, mixing, quantitative analysis, radioimmunoassays, radiolabeling
Abstract:
Scintillation proximity assay (SPA) is a type of radioimmunoassay (RIA). We apply ultrasound enhancement to the general SPA. All assay procedures, including the antibody coating and radiolabeled antigen binding are achieved by simply mixing then standing for 5 min in an ultrasound chamber. No additional incubation time is required. To further demonstrate the capability of the UE-SPA, a quantitative measurement of CD55 in various grades of colon tumors was assessed on human tissue slides. The results showed a significant correlation between CD55 expression and tumorigenesis. In conclusion, we confirmed that UE-SPA is a reliable, rapid and alternative to RIA.
Agid:
6316002