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DExH-Box helicase 58 enhances osteoblast differentiation of osteoblastic cells via Wnt/β-Catenin signaling

Zhou, Lei, Sun, Shiwei, Xu, Liang, Yu, Yueming, Zhang, Tieqi, Wang, Minghai
Biochemical and biophysical research communications 2019
RNA helicases, biomarkers, bone diseases, bone formation, genes, mice, osteoblasts, signal transduction, therapeutics
Discovering genes with regulatory effect of osteoblast differentiation and revealing mechanism of osteogenesis will enable us to find out more therapeutic methods for treating bone diseases. In this study, we supposed DHX58 may have a close relationship with osteogenesis and we then detected the expression of DHX58 during osteogenesis. We found that DHX58 was increased along with the osteogenic induction time extended. We then analyzed the function of DHX58 on ossification of mouse osteoblasts. The knockdown of DHX58 suppressed osteogenesis, as well as the expression of osteogenic biomarkers Runx2, OCN and Col1α1. Besides, the canonical Wnt/β-Catenin signaling pathway was found significant inhibited as DHX58 downregulated, indicating it's the downstream pathway of DHX58 in regulating osteogenesis. Furthermore, we overexpressed DHX58 and the results were in accordance with the above findings. Taken together, our results indicated that DHX58 promotes osteogenesis of mouse osteoblasts via the canonical Wnt/β-Catenin signaling pathway.