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Drosophila Trf4-1 involves in mRNA and primary miRNA transcription

Author:
Liu, Yongxiang, Wang, Ming, Liu, Xin, Quan, Jia, Fang, Yuan, Liu, Yujie, Qiu, Yang, Yu, Yang, Zhou, Xi
Source:
Biochemical and biophysical research communications 2019 v.511 no.4 pp. 806-812
ISSN:
0006-291X
Subject:
DNA-directed RNA polymerase, Drosophila, chromatin, cytoplasm, imagos, messenger RNA, microRNA, precipitin tests, promoter regions, small nuclear RNA, transcription (genetics)
Abstract:
Drosophila Trf4-1 (DmTrf4-1) is a polyadenylation polymerase or terminal nucleotidyl transferase (PAP/TENT) that has been reported to add poly(A) tails to snRNAs in nucleus or mRNAs in cytoplasm. Here, we found that the loss of Trf4-1 resulted in the reduction of mRNAs and primary miRNAs (pri-miRNAs) in both Drosophila S2 cells and adult flies. Interestingly, the role of Trf4-1 in transcription is independent of its PAP/TENT activity. Moreover, using the chromatin immunoprecipitation assay, we uncovered that the loss of Trf4-1 led to abnormal RNA polymerase II accumulation and reduced H3K4me3 binding in promoter regions. Thus, our study indicates a positive role of Trf4-1 in the transcription of mRNAs and pri-miRNAs.
Agid:
6316992