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A lysosome-targetable fluorescent probe for the simultaneous sensing of Cys/Hcy and GSH from different emission channels

Author:
Zhang, Hui, Xu, Lizhen, Li, Wenxiu, Chen, Wenqiang, Xiao, Qi, Huang, Jun, Huang, Chusheng, Sheng, Jiarong, Song, Xiangzhi
Source:
RSC advances 2019 v.9 no.14 pp. 7955-7960
ISSN:
2046-2069
Subject:
coumarin, emissions, fluorescent dyes, human cell lines, lysosomes, moieties, p-nitrophenol
Abstract:
A lysosome-specific fluorescent probe, Lyso-AC, for biothiols was developed by incorporation of a 4-nitrophenol moiety into a coumarin dye. The Cys/Hcy-triggered substitution-rearrangement cascade, and GSH-induced substitution reaction lead to the corresponding blue emissive amino-coumarin and yellow emissive thiol-coumarin, thereby enabling Cys/Hcy and GSH detection from distinct emissions. Moreover, this probe displayed an excellent lysosome targeting property with a 0.92 Pearson's colocalization coefficient by using Neutral Red as a reference. Significantly, biological experiments indicated Lyso-AC has the potential to monitor lysosome Cys/Hcy and GSH simultaneously in living HeLa cells from distinct emissions.
Agid:
6322598